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rat insulin enzyme linked immunosorbent assay elisa kit  (ALPCO)


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    Structured Review

    ALPCO rat insulin enzyme linked immunosorbent assay elisa kit
    Rat Insulin Enzyme Linked Immunosorbent Assay Elisa Kit, supplied by ALPCO, used in various techniques. Bioz Stars score: 98/100, based on 2397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunosorbent+assay+kit/Insulin+ELISA/pm42509746-81-7-18
    Average 98 stars, based on 2397 article reviews
    rat insulin enzyme linked immunosorbent assay elisa kit - by Bioz Stars, 2026-10
    98/100 stars

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    Article Title: A novel polyphenol-rich combination of 5 plant extracts prevents high-fat diet-induced body weight gain by regulating intestinal macronutrient absorption in mice.
    Article Snippet: Global prevalence of obesity and type 2 diabetes are rapidly increasing to pandemic proportions.. A novel supplement composed of 5 plant extracts from olive leaf, bilberry, artichoke, chrysanthellum, and black pepper was designed to prevent type 2 diabetes development in people at risk.. It was previously shown to improve body weight and glucose control in preclinical rodent models, with these effects being accompanied by increased fecal energy excretion and in vitro inhibition of several digestive enzymes.

    Article Title: Disruption of Growth Hormone Receptor in Adipocytes Improves Insulin Sensitivity and Lifespan in Mice.
    Article Snippet: Adiponectin was measured using a mouse high-molecular-weight and total adiponectin enzyme-linked immunosorbent assay kit (RRID: AB_2801466; catalog No. 47-ADPMS-E01; ALPCO) according to the manufacturer’s instructions, with 7 to 11 mice per group.

    Article Title: Increasing particle size of oat flours decreases postprandial glycemia and increases appetite in healthy adults
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Clinical Proteomics:

    Article Title: Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
    Article Snippet: Plasma glucose was determined by an enzymatic test using an automated system (Olympus AU 400 analyzer, CRI biochemistry platform). .. Plasma insulin concentrations were measured using a commercially available rat insulin enzyme-linked immunosorbent assay kit (80-INSRT-E01, Alpco Immunoassays) at each time point, and optical densities were determined at 450 nm using a Tecan Infinite M200 Pro plate reader (Tecan US, Inc). .. Data were analyzed using SAS Studio (SAS Institute, Inc) by 2- factor ANOVA and repeated measures 2-factor ANOVA, followed by the Tukey-Kramer test for post hoc comparison of means.

    Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia
    Article Snippet: NHP EDTA–plasma samples were analyzed for inflammatory biomarkers by the Endocrine Technologies Core (ETC) at the ONPRC. .. Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions. ..

    Article Title: Salbutamol Increases Leg Glucose Uptake and Metabolic Rate but not Muscle Glycogen Resynthesis in Recovery From Exercise.
    Article Snippet: .. Plasma insulin concentrations were determined for each subject with a commercially available Ultrasensitive enzyme-linked immunosorbent assay kit (ref. 80-INSHUU-E10, Alpco, NH, USA) and analyzed on a Multiskan FC microplate photometer (Thermo Fisher Scientific, MA, USA). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Novel cell-permeable p38-MAPK inhibitor efficiently prevents porcine islet apoptosis and improves islet graft function.
    Article Snippet: Briefly, porcine pancreatic islets were incubated for 6 h with or without 10 μM 11R-mp38I110 or 11R-p38I110 and 1,200 IEs was incubated with 2.8 mM or 25 mM glucose in RPMI 1640 (Nacalai Tesque, Kyoto, Japan) for 2 h at 37 °C in an atmosphere containing 5% CO2. .. The supernatants were collected, and insulin levels were determined using an enzyme-linked immunosorbent assay kit (ALPCO Insulin ELISA kit; ALPO Diagnostics, Windham, NH, USA). ..

    Article Title: Activation of coagulation FXI promotes endothelial inflammation and amplifies platelet activation in a nonhuman primate model of hyperlipidemia
    Article Snippet: NHP EDTA–plasma samples were analyzed for inflammatory biomarkers by the Endocrine Technologies Core (ETC) at the ONPRC. .. Plasma samples were analyzed for C-reactive protein using an enzyme-linked immunosorbent assay kit (ELISA, ALPCO) following the manufacturer’s instructions. ..

    Article Title: Effects of a Brown Beans Evening Meal on Metabolic Risk Markers and Appetite Regulating Hormones at a Subsequent Standardized Breakfast: A Randomized Cross-Over Study
    Article Snippet: The quantitative determination of s-IL-6 was performed with an enzyme immunoassay (Human IL-6 HS600B, R&D Systems, Abingdon, UK, and IL-18 in serum with an enzyme immunoassay that was modified in the respect that no dilution of serum was performed prior to the analysis (Human IL-18 ELISA Kit 7620, MBL Medical & Biological Laboratories CO., Ltd, Nagoya, Japan). .. Plasma adiponectin concentrations were determined with a solid phase two-site enzyme immunoassay kit (Adiponectin ELISA 10-1193-01, Mercodia AB, Uppsala, Sweden), and the quantitative determination of bioactive GLP-1 levels in plasma with a highly sensitive ELISA enzyme-linked immunosorbent assay kit (GLP-1 (Active 7–36) ELISA 43-GP1HU-EO1 ALPCO Diagnostics, Salem, NH). .. Plasma GLP-2 concentrations were determined with a competitive enzyme immunoassay (Human GLP-2 EIA YK141, Yanaihara Institute Inc. Shizuoka, Japan), p-ghrelin with an enzyme immunometric assay (Human Acylated Ghrelin ELISA RD194062400R, BioVender GmbH, Heidelberg, Germany), and p-OXM with an immunoassay (Human Oxyntomodulin ELISA Kit XSB-E12948h, Cusabio Biotech CO., Ltd, Newark, USA).



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    TIPE promotes M2 polarization through <t>DcR3.</t> A GEPIA database analysis of the correlation between TIPE and M2 marker molecules CD206, CD163, and IL-10 in CRC tissues. B Western blot verified the transfection efficiency of TIPE overexpression and knockdown plasmids in SW480 and HCT116, respectively. C and D Expression of CD206, CD163, IL-10, CD80, CD86, and IL-1β was detected by qPCR after the action of SW480-OE, and SW480-V CM on murine-derived macrophages RAW264.7. E UALCAN database analysis of DcR3 expression in CRC in situ tumors. F Expression and correlation analysis of DcR3 and TIPE in tumors and paracancerous tissues of CRC patients were detected by qPCR. G Western blot validation of DcR3 expression in TIPE differentially expressing CRC cells. H ELISA for DcR3 expression in SW480-OE, SW480-V supernatants. I GEO database analysis of DcR3, CD163 expression in CRC tissues. J GEPIA database analysis of DcR3 correlation with CD206, CD163, and IL-10 in CRC tissues. K Measurement of ARG-1 enzymatic activity in M0 macrophages following exogenous DcR3 treatment. L Expression of CD206, CD163, ARG-1, and IL-10 after exogenous DcR3 action on M0 was detected by qPCR. M Proportion of CD206, CD163, and ARG-1-positive macrophages after exogenous DcR3 action on M0 was detected by flow cytometry. N The proportion of CD206, CD163, and ARG-1-positive macrophages was detected by flow cytometry after addition of exogenous DcR3 and anti-DcR3 to SW480-V and SW480-OE CM-acting M0, respectively. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001
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    UA directly induces CD8 + T-cell exhaustion and impairs cytotoxicity. A, Representative flow cytometry plots showing IFNγ, GZMB, PD-1, and Tim-3 expression in OT-1 CD8 + T cells after 7 days of in vitro stimulation with 100 ng/mL OVA peptide, with or without UA treatment (70 μg/mL). B, Quantification of IFNγ, GZMB, PD-1, and Tim-3 expression levels corresponding to A . C, In vitro cytotoxicity assay assessing the killing efficiency of OT-1 CD8 + T cells cultured for 7 days with or without UA against CFSE-labeled MC38-OVA target cells at E:T ratios of 1:1, 2:1, 4:1, and 8:1. Target cell apoptosis was measured by active caspase-3 expression. Tim-3 expression on CD8 + T cells under each condition was also analyzed by flow cytometry. D, Quantitative analysis of killing efficiency and Tim-3 expression from C . E, qPCR analysis of Ifng and Gzmb mRNA expression in OT-1 CD8 + T cells cultured with or without UA on days 5 and 7 after activation. F, <t>ELISA</t> quantification of IFNγ and GZMB protein levels in culture supernatants from OT-1 CD8 + T cells with or without UA treatment, measured on days 5 and 7 after activation. G, Schematic of the in vivo experimental model: CD45.1 + C57BL/6J mice bearing subcutaneous MC38-OVA tumors received intratumoral adoptive transfer of CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells pretreated with or without UA ( n = 5 mice per group). H, Tumor growth curves of MC38-OVA tumors following adoptive transfer of the indicated CD8 + T-cell groups described in G . I, Final tumor weights at endpoint for each group described in G . J, Flow cytometry analysis of functional markers (IFNγ and GZMB) and exhaustion markers (PD-1 and Tim-3) in tumor-infiltrating CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells from experiments described in G . I and J, share the same legend and color codes as H . Note: for I and J , n = 3 in the Havcr2 −/− OT-1 CD8 + T group, as analysis was restricted to residual tumors; other mice achieved complete remission. Statistical significance was determined by comparison of endpoint tumor volumes/weights. Data represent mean ± SEM. Statistical significance was determined by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
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    Image Search Results


    TIPE promotes M2 polarization through DcR3. A GEPIA database analysis of the correlation between TIPE and M2 marker molecules CD206, CD163, and IL-10 in CRC tissues. B Western blot verified the transfection efficiency of TIPE overexpression and knockdown plasmids in SW480 and HCT116, respectively. C and D Expression of CD206, CD163, IL-10, CD80, CD86, and IL-1β was detected by qPCR after the action of SW480-OE, and SW480-V CM on murine-derived macrophages RAW264.7. E UALCAN database analysis of DcR3 expression in CRC in situ tumors. F Expression and correlation analysis of DcR3 and TIPE in tumors and paracancerous tissues of CRC patients were detected by qPCR. G Western blot validation of DcR3 expression in TIPE differentially expressing CRC cells. H ELISA for DcR3 expression in SW480-OE, SW480-V supernatants. I GEO database analysis of DcR3, CD163 expression in CRC tissues. J GEPIA database analysis of DcR3 correlation with CD206, CD163, and IL-10 in CRC tissues. K Measurement of ARG-1 enzymatic activity in M0 macrophages following exogenous DcR3 treatment. L Expression of CD206, CD163, ARG-1, and IL-10 after exogenous DcR3 action on M0 was detected by qPCR. M Proportion of CD206, CD163, and ARG-1-positive macrophages after exogenous DcR3 action on M0 was detected by flow cytometry. N The proportion of CD206, CD163, and ARG-1-positive macrophages was detected by flow cytometry after addition of exogenous DcR3 and anti-DcR3 to SW480-V and SW480-OE CM-acting M0, respectively. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: TIPE promotes M2 polarization through DcR3. A GEPIA database analysis of the correlation between TIPE and M2 marker molecules CD206, CD163, and IL-10 in CRC tissues. B Western blot verified the transfection efficiency of TIPE overexpression and knockdown plasmids in SW480 and HCT116, respectively. C and D Expression of CD206, CD163, IL-10, CD80, CD86, and IL-1β was detected by qPCR after the action of SW480-OE, and SW480-V CM on murine-derived macrophages RAW264.7. E UALCAN database analysis of DcR3 expression in CRC in situ tumors. F Expression and correlation analysis of DcR3 and TIPE in tumors and paracancerous tissues of CRC patients were detected by qPCR. G Western blot validation of DcR3 expression in TIPE differentially expressing CRC cells. H ELISA for DcR3 expression in SW480-OE, SW480-V supernatants. I GEO database analysis of DcR3, CD163 expression in CRC tissues. J GEPIA database analysis of DcR3 correlation with CD206, CD163, and IL-10 in CRC tissues. K Measurement of ARG-1 enzymatic activity in M0 macrophages following exogenous DcR3 treatment. L Expression of CD206, CD163, ARG-1, and IL-10 after exogenous DcR3 action on M0 was detected by qPCR. M Proportion of CD206, CD163, and ARG-1-positive macrophages after exogenous DcR3 action on M0 was detected by flow cytometry. N The proportion of CD206, CD163, and ARG-1-positive macrophages was detected by flow cytometry after addition of exogenous DcR3 and anti-DcR3 to SW480-V and SW480-OE CM-acting M0, respectively. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: Analysis, Marker, Western Blot, Transfection, Over Expression, Knockdown, Expressing, Derivative Assay, In Situ, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Activity Assay, Flow Cytometry

    DcR3 exerts its function via binding to HSPG2 in an HS-dependent manner. A KEGG pathway enrichment in RNA-Seq sequencing results. B The volcano plot represented the expression of HSPG2 in the RNA-Seq sequencing results. C qPCR detection of HSPG2 expression in tumors and paracancerous tissues of CRC patients and analysis of correlation with TIPE. D qPCR was performed to detect the expression of HSPG2 after SW480-OE, SW480-V, HCT116-V, and HCT116-KD CM action on M0. E Western blot detection of HSPG2 expression after SW480-OE, SW480-V CM-acting M0. F Endotoxin of exogenous DcR3 was measured via the turbidimetric limulus test. DcR3 was prepared at a concentration of 0.55 mg/mL, and applied at 5.5 μg/mL. G qPCR detection of HSPG2 expression after exogenous DcR3 acting M0. H Western blot detection of HSPG2 expression after addition of exogenous DcR3, anti-DcR3 to SW480-V, SW480-OE CM-acting M0, respectively. I PyMOL predicts DcR3 and HSPG2 binding sites. J Flow cytometry was used to detect the surface expression of HSPG2. K ELISA was used to detect DcR3 levels in cell culture supernatants. Samples were collected from M0 macrophages treated with DcR3 for 24 h, with or without heparin sodium (HS) or heparinase III pretreatment. L Western blot was used to detect p-PI3K and p-AKT levels. M0 macrophages were treated with heparin sodium (HS) or heparinase III, followed by incubation with DcR3 for 24 h. M Immunofluorescence detection of the colocalization between DcR3 and HSPG2. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: DcR3 exerts its function via binding to HSPG2 in an HS-dependent manner. A KEGG pathway enrichment in RNA-Seq sequencing results. B The volcano plot represented the expression of HSPG2 in the RNA-Seq sequencing results. C qPCR detection of HSPG2 expression in tumors and paracancerous tissues of CRC patients and analysis of correlation with TIPE. D qPCR was performed to detect the expression of HSPG2 after SW480-OE, SW480-V, HCT116-V, and HCT116-KD CM action on M0. E Western blot detection of HSPG2 expression after SW480-OE, SW480-V CM-acting M0. F Endotoxin of exogenous DcR3 was measured via the turbidimetric limulus test. DcR3 was prepared at a concentration of 0.55 mg/mL, and applied at 5.5 μg/mL. G qPCR detection of HSPG2 expression after exogenous DcR3 acting M0. H Western blot detection of HSPG2 expression after addition of exogenous DcR3, anti-DcR3 to SW480-V, SW480-OE CM-acting M0, respectively. I PyMOL predicts DcR3 and HSPG2 binding sites. J Flow cytometry was used to detect the surface expression of HSPG2. K ELISA was used to detect DcR3 levels in cell culture supernatants. Samples were collected from M0 macrophages treated with DcR3 for 24 h, with or without heparin sodium (HS) or heparinase III pretreatment. L Western blot was used to detect p-PI3K and p-AKT levels. M0 macrophages were treated with heparin sodium (HS) or heparinase III, followed by incubation with DcR3 for 24 h. M Immunofluorescence detection of the colocalization between DcR3 and HSPG2. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: Binding Assay, RNA Sequencing, Sequencing, Expressing, Analysis, Western Blot, Concentration Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Cell Culture, Incubation, Immunofluorescence

    DcR3–HSPG2 complex activates PI3K, followed by the recruitment and activation of AKT1. A KEGG pathway enrichment in RNA-Seq sequencing results. B The volcano plot represented the expression of AKT1 in the RNA-Seq sequencing results. C Western blot was used to detect the expression of AKT1 and p-AKT1 after SW480-OE and SW480-V CM action on M0. D qPCR detection of AKT1 expression after exogenous DcR3 action on M0. E Western blot detected the expression of AKT1 and p-AKT1 after addition of exogenous DcR3 and anti-DcR3 to SW480-V and SW480-OE CM following acting on M0, respectively. F The GEPIA database was analyzed for HSPG2 and AKT1 correlation. G After applying siRNA to silence HSPG2 expression, qPCR was performed to detect AKT1 expression. H Western blot analysis of AKT1, p-AKT1, PI3K, and p-PI3K expression following siRNA-mediated silencing of HSPG2. I Membrane protein extraction and Western blot detection of Na⁺/K⁺-ATPase and AKT1 in HSPG2-silenced macrophages stimulated with exogenous DcR3. J Western blot analysis of AKT1, p-AKT1, PI3K, and p-PI3K expression in macrophages pretreated with the PI3K-specific inhibitor LY294002. K PyMOL predicts potential interaction regions between HSPG2 and AKT1. L Co-immunoprecipitation (Co-IP) assay demonstrating the physical interaction between HSPG2 and AKT1. M Immunofluorescence assay showing the colocalization of DcR3 and HSPG2. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: DcR3–HSPG2 complex activates PI3K, followed by the recruitment and activation of AKT1. A KEGG pathway enrichment in RNA-Seq sequencing results. B The volcano plot represented the expression of AKT1 in the RNA-Seq sequencing results. C Western blot was used to detect the expression of AKT1 and p-AKT1 after SW480-OE and SW480-V CM action on M0. D qPCR detection of AKT1 expression after exogenous DcR3 action on M0. E Western blot detected the expression of AKT1 and p-AKT1 after addition of exogenous DcR3 and anti-DcR3 to SW480-V and SW480-OE CM following acting on M0, respectively. F The GEPIA database was analyzed for HSPG2 and AKT1 correlation. G After applying siRNA to silence HSPG2 expression, qPCR was performed to detect AKT1 expression. H Western blot analysis of AKT1, p-AKT1, PI3K, and p-PI3K expression following siRNA-mediated silencing of HSPG2. I Membrane protein extraction and Western blot detection of Na⁺/K⁺-ATPase and AKT1 in HSPG2-silenced macrophages stimulated with exogenous DcR3. J Western blot analysis of AKT1, p-AKT1, PI3K, and p-PI3K expression in macrophages pretreated with the PI3K-specific inhibitor LY294002. K PyMOL predicts potential interaction regions between HSPG2 and AKT1. L Co-immunoprecipitation (Co-IP) assay demonstrating the physical interaction between HSPG2 and AKT1. M Immunofluorescence assay showing the colocalization of DcR3 and HSPG2. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: Activation Assay, RNA Sequencing, Sequencing, Expressing, Western Blot, Analysis, Membrane, Protein Extraction, Co-Immunoprecipitation Assay, Immunofluorescence

    TIPE promotes macrophage proliferation and migration via DcR3. A SW480, HCT116 CM were all mixed with DcR3 and anti-DcR3 to act on Mφ, respectively, and CCK-8 detected the proliferation of THP-1 ( B ) Scratch wound assay detecting RAW264.7 cell migration. Macrophages were treated with SW480 or HCT116 conditioned medium (CM) supplemented with exogenous DcR3 or anti-DcR3, respectively. C Transwell detection of RAW264.7 infiltration after SW480-V, SW480-OE, and exogenous DcR3 alone act on Mφ. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: TIPE promotes macrophage proliferation and migration via DcR3. A SW480, HCT116 CM were all mixed with DcR3 and anti-DcR3 to act on Mφ, respectively, and CCK-8 detected the proliferation of THP-1 ( B ) Scratch wound assay detecting RAW264.7 cell migration. Macrophages were treated with SW480 or HCT116 conditioned medium (CM) supplemented with exogenous DcR3 or anti-DcR3, respectively. C Transwell detection of RAW264.7 infiltration after SW480-V, SW480-OE, and exogenous DcR3 alone act on Mφ. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: Migration, CCK-8 Assay, Scratch Wound Assay Assay

    M2 promotes CRC proliferation and migration. A GEPIA database analysis of the correlation between TIPE, DcR3 and CXCL8 in CRC tissues. B qPCR was performed to detect the expression of CXCL8 after SW480-OE, SW480-V CM and exogenous DcR3 acting on M0. C and D Kaplan–Meier plotter database to analyze the relationship between the expression of CD206, CD163, ARG-1, IL-10, HSPG2, and AKT1 and the prognosis of CRC patients. E and F The effect of M2 CM on SW480-OE proliferation was detected by live cell counting and CCK-8. G Transwell migration assay detecting the regulatory effect of M2 macrophages on SW480-OE cell migration. H Scratch experiments were performed to detect the effect of M2 CM on SW480-OE migration. I qPCR was performed to detect CXCR1/2 expression in SW480-OE, SW480-V. J qPCR detection of CXCR1/2 expression in SW480-OE after siRNA-CXCR1/2 transfection. K Cell counting and CCK-8 assays detected the effect of M2 CM on SW480-OE proliferation after siRNA-CXCR1/2 transfection. L Transwell migration assay detecting the effect of M2 macrophages on the migration of SW480-OE cells transfected with siRNA-CXCR1/2. M Scratch assay to detect the effect of M2 CM on SW480-OE migration after siRNA-CXCR1/2 transfection. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: M2 promotes CRC proliferation and migration. A GEPIA database analysis of the correlation between TIPE, DcR3 and CXCL8 in CRC tissues. B qPCR was performed to detect the expression of CXCL8 after SW480-OE, SW480-V CM and exogenous DcR3 acting on M0. C and D Kaplan–Meier plotter database to analyze the relationship between the expression of CD206, CD163, ARG-1, IL-10, HSPG2, and AKT1 and the prognosis of CRC patients. E and F The effect of M2 CM on SW480-OE proliferation was detected by live cell counting and CCK-8. G Transwell migration assay detecting the regulatory effect of M2 macrophages on SW480-OE cell migration. H Scratch experiments were performed to detect the effect of M2 CM on SW480-OE migration. I qPCR was performed to detect CXCR1/2 expression in SW480-OE, SW480-V. J qPCR detection of CXCR1/2 expression in SW480-OE after siRNA-CXCR1/2 transfection. K Cell counting and CCK-8 assays detected the effect of M2 CM on SW480-OE proliferation after siRNA-CXCR1/2 transfection. L Transwell migration assay detecting the effect of M2 macrophages on the migration of SW480-OE cells transfected with siRNA-CXCR1/2. M Scratch assay to detect the effect of M2 CM on SW480-OE migration after siRNA-CXCR1/2 transfection. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: Migration, Analysis, Expressing, Cell Counting, CCK-8 Assay, Transwell Migration Assay, Transfection, Scratch Assay

    In vivo experiments have confirmed that TIPE promotes M2 polarization and CRC proliferation through DcR3. A Schematic diagram of nude mouse inoculation. B Changes in body weight of mice. C Tumor volume statistics. D IHC detection of Ki-67 expression in CRC tumor tissues. E – G Proportion of CD86, CD206, CD163, and ARG-1-positive macrophages in tumor tissues, paraneoplastic lymph nodes, and spleen were detected by flow cytometry. H Expression of HSPG2, AKT1, and CXCL8 in CRC tumor tissues was detected by qPCR. I Western blot detection of HSPG2, AKT1, p-AKT1 expression in CRC tumor tissues. J IHC detection of p-AKT1 expression in CRC tumor tissues. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Cancer Immunology, Immunotherapy : CII

    Article Title: The effect and mechanism of TIPE in promoting M2 polarization through DcR3 in the microenvironment of colorectal cancer

    doi: 10.1007/s00262-026-04531-8

    Figure Lengend Snippet: In vivo experiments have confirmed that TIPE promotes M2 polarization and CRC proliferation through DcR3. A Schematic diagram of nude mouse inoculation. B Changes in body weight of mice. C Tumor volume statistics. D IHC detection of Ki-67 expression in CRC tumor tissues. E – G Proportion of CD86, CD206, CD163, and ARG-1-positive macrophages in tumor tissues, paraneoplastic lymph nodes, and spleen were detected by flow cytometry. H Expression of HSPG2, AKT1, and CXCL8 in CRC tumor tissues was detected by qPCR. I Western blot detection of HSPG2, AKT1, p-AKT1 expression in CRC tumor tissues. J IHC detection of p-AKT1 expression in CRC tumor tissues. Data were drawn as mean ± SD (n ≥ 3). * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: ELISA was performed using the DcR3 enzyme-linked immunosorbent assay kit (RayBiotech, USA) according to the manufacturer’s protocol.

    Techniques: In Vivo, Expressing, Flow Cytometry, Western Blot

    UA directly induces CD8 + T-cell exhaustion and impairs cytotoxicity. A, Representative flow cytometry plots showing IFNγ, GZMB, PD-1, and Tim-3 expression in OT-1 CD8 + T cells after 7 days of in vitro stimulation with 100 ng/mL OVA peptide, with or without UA treatment (70 μg/mL). B, Quantification of IFNγ, GZMB, PD-1, and Tim-3 expression levels corresponding to A . C, In vitro cytotoxicity assay assessing the killing efficiency of OT-1 CD8 + T cells cultured for 7 days with or without UA against CFSE-labeled MC38-OVA target cells at E:T ratios of 1:1, 2:1, 4:1, and 8:1. Target cell apoptosis was measured by active caspase-3 expression. Tim-3 expression on CD8 + T cells under each condition was also analyzed by flow cytometry. D, Quantitative analysis of killing efficiency and Tim-3 expression from C . E, qPCR analysis of Ifng and Gzmb mRNA expression in OT-1 CD8 + T cells cultured with or without UA on days 5 and 7 after activation. F, ELISA quantification of IFNγ and GZMB protein levels in culture supernatants from OT-1 CD8 + T cells with or without UA treatment, measured on days 5 and 7 after activation. G, Schematic of the in vivo experimental model: CD45.1 + C57BL/6J mice bearing subcutaneous MC38-OVA tumors received intratumoral adoptive transfer of CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells pretreated with or without UA ( n = 5 mice per group). H, Tumor growth curves of MC38-OVA tumors following adoptive transfer of the indicated CD8 + T-cell groups described in G . I, Final tumor weights at endpoint for each group described in G . J, Flow cytometry analysis of functional markers (IFNγ and GZMB) and exhaustion markers (PD-1 and Tim-3) in tumor-infiltrating CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells from experiments described in G . I and J, share the same legend and color codes as H . Note: for I and J , n = 3 in the Havcr2 −/− OT-1 CD8 + T group, as analysis was restricted to residual tumors; other mice achieved complete remission. Statistical significance was determined by comparison of endpoint tumor volumes/weights. Data represent mean ± SEM. Statistical significance was determined by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Journal: Cancer Research

    Article Title: Soluble Uric Acid Drives CD8 + T-cell Exhaustion by Inducing KSR1-Mediated MAPK Hyperactivation

    doi: 10.1158/0008-5472.CAN-25-3911

    Figure Lengend Snippet: UA directly induces CD8 + T-cell exhaustion and impairs cytotoxicity. A, Representative flow cytometry plots showing IFNγ, GZMB, PD-1, and Tim-3 expression in OT-1 CD8 + T cells after 7 days of in vitro stimulation with 100 ng/mL OVA peptide, with or without UA treatment (70 μg/mL). B, Quantification of IFNγ, GZMB, PD-1, and Tim-3 expression levels corresponding to A . C, In vitro cytotoxicity assay assessing the killing efficiency of OT-1 CD8 + T cells cultured for 7 days with or without UA against CFSE-labeled MC38-OVA target cells at E:T ratios of 1:1, 2:1, 4:1, and 8:1. Target cell apoptosis was measured by active caspase-3 expression. Tim-3 expression on CD8 + T cells under each condition was also analyzed by flow cytometry. D, Quantitative analysis of killing efficiency and Tim-3 expression from C . E, qPCR analysis of Ifng and Gzmb mRNA expression in OT-1 CD8 + T cells cultured with or without UA on days 5 and 7 after activation. F, ELISA quantification of IFNγ and GZMB protein levels in culture supernatants from OT-1 CD8 + T cells with or without UA treatment, measured on days 5 and 7 after activation. G, Schematic of the in vivo experimental model: CD45.1 + C57BL/6J mice bearing subcutaneous MC38-OVA tumors received intratumoral adoptive transfer of CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells pretreated with or without UA ( n = 5 mice per group). H, Tumor growth curves of MC38-OVA tumors following adoptive transfer of the indicated CD8 + T-cell groups described in G . I, Final tumor weights at endpoint for each group described in G . J, Flow cytometry analysis of functional markers (IFNγ and GZMB) and exhaustion markers (PD-1 and Tim-3) in tumor-infiltrating CD45.2 + WT or Havcr2 −/− OT-1 CD8 + T cells from experiments described in G . I and J, share the same legend and color codes as H . Note: for I and J , n = 3 in the Havcr2 −/− OT-1 CD8 + T group, as analysis was restricted to residual tumors; other mice achieved complete remission. Statistical significance was determined by comparison of endpoint tumor volumes/weights. Data represent mean ± SEM. Statistical significance was determined by one-way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

    Article Snippet: Mouse IFNγ and GZMB concentrations in supernatants were quantified using commercial enzyme-linked immunosorbent assay (ELISA) kits (ABclonal) according to the manufacturer’s protocol.

    Techniques: Flow Cytometry, Expressing, In Vitro, Cytotoxicity Assay, Cell Culture, Labeling, Activation Assay, Enzyme-linked Immunosorbent Assay, In Vivo, Adoptive Transfer Assay, Functional Assay, Comparison